Antimicrobial activity of Mul-1867, a novel antimicrobial compound, against multidrug-resistant Pseudomonas aeruginosa

Annals of Clinical Microbiology and Antimicrobials (2016)

Antimicrobial activity of Mul-1867, a novel antimicrobial compound, against multidrug-resistant Pseudomonas aeruginosa

Publication Type  Journal Article
 Authors George Tetz
Daria Vikina
Victor Tetz
 Abstract  Background

There is an urgent need for new antimicrobial compounds to treat various lung infections caused by multidrug-resistant Pseudomonas aeruginosa (MDR-PA).

Methods

We studied the potency of Mul-1867 against MDR-PA isolates from patients with cystic fibrosis, chronic obstructive pulmonary disease, and ventilator-associated pneumonia. The minimal inhibitory concentrations (MICs) and minimum biofilm eliminating concentrations (MBECs), defined as the concentrations of drug that kill 50 % (MBEC50), 90 % (MBEC90), and 100 % (MBEC100) of the bacteria in preformed biofilms, were determined by using the broth macrodilution method.

Results

Mul-1867 exhibited significant activity against MDR-PA and susceptible control strains, with MICs ranging from 1.0 to 8.0 µg/mL. Mul-1867 also possesses anti-biofilm activity against mucoid and non-mucoid 24-h- old MDR-PA biofilms. The MBEC50 value was equal to onefold the MIC. The MBEC90 value ranged from two to fourfold the MIC. Moreover, Mul-1867 completely eradicated mature biofilms at the concentrations tested, with MBEC100 values ranging between 16- and 32-fold the MIC. Mul-1867 was non-toxic to Madin-Darby canine kidney (MDCK) cells at concentrations up to 256 µg/mL.

Conclusion

Overall, these data indicate that Mul-1867 is a promising locally acting antimicrobial for the treatment and prevention of P. aeruginosa infections.

Year of
Publication
 2016
 Journal  Annals of Clinical Microbiology and Antimicrobials
 DOI 10.1186/s12941-016-0134-4