|Genus||Bacillussp. VT 712|
We have sequenced and analyzed the genome of the previously unculturable clinical isolateBacillus sp. VT 712 ,gram-positive, spore-forming, rod-shaped, aerobic bacteriathat was isolated from the duodenum of a patient with intestinal cancerer.
Bacillus sp. VT 712 is aerobic, motile, gram-positive bacilli.
Bacillus sp. VT 712 is a member of the family Bacillaceae, which consists of a variety of medically important species that are associated with numerous pathologies such as diarrheal syndromes, anthrax, pneumonia, sepsis, nervous system infections, particularly in immunosuppressed hosts. The genetic analyses of Bacillus sp. VT 712 identifieda significant number of antibiotic resistance genes, including those encoding resistance to vancomycin, tetracycline and fosfomycin, as well as genes of multidrug resistance efflux pumps. The analysis allowed the identification of genesencoding resistance to the chemotherapeutic drug daunorubicin, which was previously used in the patient.
The genome also contains previously known virulence factors, such as hemolysin D, flagellar, and chemotaxis proteins, and numerous peptidases and ureases .
Bacillus sp. VT 712 spores are highly resistant to heat, antibiotics, and chemicals.
Nucleotide sequence accession number
This genome sequencing project has been deposited in GenBank under the accession LWBK00000000.